Concepts
Overview states what Nornir is for. Overview of volume building walks through mosaic capture, slice-to-slice registration, and slice-to-volume mapping, and names the nornir-build command for each stage.
Glossary
- Block
Plastic-embedded sample before sectioning. In the volume tree, a block groups sections and is the unit of batch processing.
- Section / slice
One thin physical slice from the block, typically tens of nanometres thick for TEM. One Z level in the volume.
- Channel
A named image set on a section, such as
TEMor a derived filter stack.- Filter
An image-processing product of a channel (raw tiles, leveled tiles, a blob image). Pipelines select filters by name.
- Tile
One microscope image in a mosaic. Neighbors overlap on purpose.
- Mosaic
The set of tiles that covers an area larger than one field of view, plus the transform that lays them out. Stored as a
.mosaicfile.- Pyramid level
A downsampled copy of a tile or assembled image. Lower levels are smaller and are what registration usually measures.
- Prune
Drop tiles that do not have enough texture to align. The
Prunepipeline does this beforeMosaic.- Slice-to-slice (STOS)
A transform that aligns one section to an adjacent section. Stored as a
.stosfile.- Blob
A texture filter that keeps features visible after downsampling.
CreateBlobFilterbuilds it. TEM brute alignment uses it.- Grid / mesh
After a rigid or translation step, a grid of cells is measured and the mesh records a local offset at each cell. Mosaic refine and slice-to-slice refine both do this. In Pyre, a refined grid does not support adding or deleting control points the way a triangulation transform does.
- Center section
The section chosen as the origin of the volume coordinate system. Other sections map toward it.
- Slice-to-volume
The composed mapping from a section’s mosaic space into that shared volume space.
SliceToVolumebuilds it from the slice-to-slice chain.- VikingXML
The manifest
CreateVikingXMLwrites so Viking can open the volume. TheStosGroupname is the label in Viking’s transform menu.